I wanna be a biopunk!

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GenesForLife
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Re: I wanna be a biopunk!

Post by GenesForLife » Sun Aug 22, 2010 11:56 am

Dory wrote:
GenesForLife wrote:
Dory wrote:I've thought a bit about it, and I don't really want to invest that much in lab-experiment-biopunking (which frankly doesn't even seem that real). I'd like to make a humble mini-lab though. Any relatively cheap and not-huge stuff I can buy that would be useful individually (like microscopes?) that you can think of?
Nutrient agar, Petri plates, chicken incubator, Gram's staining kit ( Safranine, Crystal Violet, Gram's Iodine, 95% ethanol (which you can make from absoulute alcohol) and distilled water), inoculation loops and needles. You can have great fun growing bacteria and fungi, staining, mounting and taking micrographs of them. Won't cost much at all.
Awesome. Thanks a bunch :D

But wait a second, why a CHICKEN incubator? Are you saying I should focus my self-research-learning on chickens?
Because that can be adapted easily for microbial cultures.

Dory
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Re: I wanna be a biopunk!

Post by Dory » Sun Aug 22, 2010 11:57 am

And by the way, are there any other cool electrical machines other than microscopes that I can use individually to research, that don't take too much room (like the CO2 incubator which you suggested which is HUGE!!!)?

You know what I really like? Colony counters! I might buy one.

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Re: I wanna be a biopunk!

Post by Dory » Sun Aug 22, 2010 11:58 am

GenesForLife wrote:
Dory wrote:
GenesForLife wrote:
Dory wrote:I've thought a bit about it, and I don't really want to invest that much in lab-experiment-biopunking (which frankly doesn't even seem that real). I'd like to make a humble mini-lab though. Any relatively cheap and not-huge stuff I can buy that would be useful individually (like microscopes?) that you can think of?
Nutrient agar, Petri plates, chicken incubator, Gram's staining kit ( Safranine, Crystal Violet, Gram's Iodine, 95% ethanol (which you can make from absoulute alcohol) and distilled water), inoculation loops and needles. You can have great fun growing bacteria and fungi, staining, mounting and taking micrographs of them. Won't cost much at all.
Awesome. Thanks a bunch :D

But wait a second, why a CHICKEN incubator? Are you saying I should focus my self-research-learning on chickens?
Because that can be adapted easily for microbial cultures.
Cool!

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Re: I wanna be a biopunk!

Post by Feck » Sun Aug 22, 2010 11:59 am

To keep cultures warm .....although If you want to do developmental biology /embryology eggs is the thing :tup:
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Re: I wanna be a biopunk!

Post by GenesForLife » Sun Aug 22, 2010 12:01 pm

Dory wrote:And by the way, are there any other cool electrical machines other than microscopes that I can use individually to research, that don't take too much room (like the CO2 incubator which you suggested which is HUGE!!!)?

You know what I really like? Colony counters! I might buy one.
You can make a Quebec colony counter using nothing more than cardboard, ink, and a magnifying glass.

Image

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Re: I wanna be a biopunk!

Post by Feck » Sun Aug 22, 2010 1:32 pm

Or just do some dilutions before you plate them up and count smaller numbers ????
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Re: I wanna be a biopunk!

Post by Feck » Sun Aug 22, 2010 1:37 pm

Colony counters, PCR machines FFS you are so spoilt nowadays :lay: tell me there is still some manual skill left in filling the wells in electrophoresis gels ?or is that done by machine also :nono:
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Re: I wanna be a biopunk!

Post by Dory » Sun Aug 22, 2010 1:46 pm

I'll by everything on Genes last list and colony counter (yes, I may be able to make them, but they look soooooo cool!). Now the big question is where from. I see only one good lab equipment supplier in my country....hmm....and you have to do it by phone. Darn it. Wish we had a store for that sorta shit.

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Re: I wanna be a biopunk!

Post by Feck » Sun Aug 22, 2010 1:59 pm

Dory wrote:I'll by everything on Genes last list and colony counter (yes, I may be able to make them, but they look soooooo cool!). Now the big question is where from. I see only one good lab equipment supplier in my country....hmm....and you have to do it by phone. Darn it. Wish we had a store for that sorta shit.
Dory you have too much money !
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Re: I wanna be a biopunk!

Post by Dory » Sun Aug 22, 2010 2:06 pm

Feck wrote:
Dory wrote:I'll by everything on Genes last list and colony counter (yes, I may be able to make them, but they look soooooo cool!). Now the big question is where from. I see only one good lab equipment supplier in my country....hmm....and you have to do it by phone. Darn it. Wish we had a store for that sorta shit.
Dory you have too much money !
living with parents can have advantages :biggrin:

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Re: I wanna be a biopunk!

Post by Feck » Sun Aug 22, 2010 3:13 pm

Dory wrote:
Feck wrote:
Dory wrote:I'll by everything on Genes last list and colony counter (yes, I may be able to make them, but they look soooooo cool!). Now the big question is where from. I see only one good lab equipment supplier in my country....hmm....and you have to do it by phone. Darn it. Wish we had a store for that sorta shit.
Dory you have too much money !
living with parents can have advantages :biggrin:
Can I suggest spending some money on books first ?
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Give me the wine , I don't need the bread

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GenesForLife
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Re: I wanna be a biopunk!

Post by GenesForLife » Sun Aug 22, 2010 4:16 pm

Feck wrote:Colony counters, PCR machines FFS you are so spoilt nowadays :lay: tell me there is still some manual skill left in filling the wells in electrophoresis gels ?or is that done by machine also :nono:
I used a Quebec colony counter mate, manual work, lots of it, haven't done PCR and the three times I've done electrophoresis I used the good old comb and micropippetors.

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Re: I wanna be a biopunk!

Post by Feck » Sun Aug 22, 2010 4:32 pm

GenesForLife wrote:
Feck wrote:Colony counters, PCR machines FFS you are so spoilt nowadays :lay: tell me there is still some manual skill left in filling the wells in electrophoresis gels ?or is that done by machine also :nono:
I used a Quebec colony counter mate, manual work, lots of it, haven't done PCR and the three times I've done electrophoresis I used the good old comb and micropippetors.
Good :biggrin:
:hoverdog: :hoverdog: :hoverdog: :hoverdog:
Give me the wine , I don't need the bread

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GenesForLife
Bertie Wooster
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Re: I wanna be a biopunk!

Post by GenesForLife » Sun Aug 22, 2010 4:35 pm

Feck wrote:
GenesForLife wrote:
Feck wrote:Colony counters, PCR machines FFS you are so spoilt nowadays :lay: tell me there is still some manual skill left in filling the wells in electrophoresis gels ?or is that done by machine also :nono:
I used a Quebec colony counter mate, manual work, lots of it, haven't done PCR and the three times I've done electrophoresis I used the good old comb and micropippetors.
Good :biggrin:
For most of my lab experiments even micropipettes weren't available, had to use those good old glass pipettes in combination with oral suction, even if that is a strict safety no-no.

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Re: I wanna be a biopunk!

Post by Feck » Sun Aug 22, 2010 4:48 pm

GenesForLife wrote:
Feck wrote:
GenesForLife wrote:
Feck wrote:Colony counters, PCR machines FFS you are so spoilt nowadays :lay: tell me there is still some manual skill left in filling the wells in electrophoresis gels ?or is that done by machine also :nono:
I used a Quebec colony counter mate, manual work, lots of it, haven't done PCR and the three times I've done electrophoresis I used the good old comb and micropippetors.
Good :biggrin:
For most of my lab experiments even micropipettes weren't available, had to use those good old glass pipettes in combination with oral suction, even if that is a strict safety no-no.
It's ok for chemistry but filling gels you need one of these Image
:hoverdog: :hoverdog: :hoverdog: :hoverdog:
Give me the wine , I don't need the bread

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