Dory wrote:GenesForLife wrote:Dory wrote:Hmm, I expected it to determine on many factors, though I did wonder what's the approximate distribution. 15 seconds for 300? Cool. Not as fast as I thought these babies normally go
The reaction to add each amino acid is a multi-step one, Dory... you need ratcheting, incorporation of aminoacyl tRNA, and the formation of peptidyl transferase, while involving GTP cleavage and the involvement of Elongation Factors Tu and G, of course, you also need to eject the empty uncharged tRNA in the E site of the ribosome.
And does the empty uncharged tRNA go to the cytoplasm to get hooked up with its correlating amino acid?
Until it is degraded, it is free to be recycled...
I have this abstract of a study on rats...
The tRNA fraction, extracted from very high speed supernatant fluid, from livers of rats injected with 3H-orotic acid, attained maximum specific activity after a little over 24 hr and, thereafter, decayed with an apparent half life of 5 days. This behaviour of tRNA was indistinguishable from that of liver rRNA and the acid soluble pool. Chromatography of tRNA, doubly labelled during a period of short synthesis and of prolonged decay, on a BD cellulose column, indicated that individual tRNA species turn over at a constant rate with respect to one another.
http://www.sciencedirect.com/science?_o ... 5bb709b527
AFAIK, there is no "locking" or "blocking step" involved in translation, the logical answer would be that reuse until degradation has an evolutionary advantage and this could be expected (think reduced transcription costs in not having to make said tRNA afresh) , mRNA, however, I suspect, has a very short half-life, and is degraded quickly after translation.